Pollution is a planetary concern which has impacted the environment and for which sustainable solution is being pursued but yet to be proffered. It is a phenomenon that has invariably evolved disputing jobs to adult male and his environment. Pollution is defined as the “ debut by adult male into environment of substances or energy apt to do jeopardies to human wellness, injury to living resources and ecological systems, harm to constructions and agreeableness or interfere with legitimate utilizations of the environment ” ( Holdgate, 1980 ) . This implies that there is an anthropogenetic alteration of the environment that could precipitate inauspicious effects to both biological and physical systems with a attendant wellness and economic impact. The aquatic environment has received a great toxic impact because it constitutes a sink or reservoir for discharge of industrial, agricultural and domestic wastes. Ecotoxicology is a field of toxicology that has evolved to set about surveies on the toxic effects of chemicals upon the ecosystem ( Walker et al. , 2001 ) . These surveies are aimed at bettering the environment and doing it more sustainable for descendants.
This survey is sing an country within the Niger Delta Nigeria as instance survey because of the immense anthropogenetic impacts ensuing from hydrocarbon geographic expedition and other industrial activities within the part. These activities present a complex environmental challenge that includes decease and hinderance to marine life, disease to worlds who depend on these aquatic nutrient beginning and decrease in aesthetic value of the aquatic environment with a attendant hinderance to recreational use. The Ovia River has been chosen peculiarly because of its fishing activities which records high as a beginning of support and career for people from the Ovia community and across boundary line. Despite the economic benefits of this river, there is record of industrial wastewater, agricultural and domestic waste discharge in to the river ( Oguzie and Achegbulu, 2010 ) .
Aquatic life is vulnerable to a broad array of anthropogenetic contaminations whose diverseness makes it about impossible to find by H2O analysis. Furthermore, these chemicals can interact in a mode that is interactive to arouse effects which is uncharacteristic of a individual chemical ( Kime, 1998 ) . In a command to besieging this challenge, certain beings have been found suited as lookout beings. Fish has been used extensively in ecotoxicological hazard appraisal modeling and has been proposed for usage in this survey because it is robust and has the intrinsic features of lookout beings used as bioindicators for environmental biomonitoring. Such features includes good indexs of medium and long term effects of the aquatic conditions, are representative of a scope of trophic degree such that a biomagnification of toxic contaminations reflects the wellness impact of the aquatic community, show unambiguous eating scheme and metamorphosis, ability to roll up and concentrate contaminations within their tissue to level that are comparatively higher than their environment, have high birthrate rate, easy to try, roll up, place and hive away ( Plafkin et al. , 1989 ) . In add-on to its hardiness as a lookout being, it besides has a great economic and nutritionary significance and enjoys relevant policy direction and determinations.
The aforesaid anthropogenetic consequence observed in the Ovia River is traceable to petroleum industrial wastewaters, agricultural and domestic wastes ( Oguzie and Achegbulu, 2010 ) . Contaminants from each of the mentioned beginnings have their distinguishable features which by and large impacts the aquatic home grounds and the inhabiting species. Most of these crude oil and agricultural contaminations are endocrinal disruptors, neurotoxic and genotoxic compounds. Some of these compounds include pesticides, polychlorinated biphenyls, polyaromatic hydrocarbons and heavy metals ( Walker et al. , 2001 ) . The temporal and spacial temperaments of these contaminations in aquatic environment are mostly determined by their physic-chemical belongingss such as solubility, divider coefficients, responsiveness, biodegradability, continuity and bioaccumulative inclination ( Holdgate, 1980 ) .
Surveies have demonstrated that these poisons accumulate and are localized within the tissues of fish such as musculus, gills, liver and sex glands ( Kime, 1998 ) . There is demand to measure toxicity in a manner that will associate dosage or concentration exposed to, clip and season of exposure and the consequent cell or tissue pathology of the lookout being under probe so as to hold an apprehension of the consequence of these pollutants on the aquatic wellness.
In making this, some “ protective and non-protective ” biochemical and physiological responses serves as terminal points to these chemical abuses received. The biochemical and physiological responses by beings to aquatic pollutants could be specific or non-specific. For illustration, the initiation of the monooxygenase within a tissue is an grounds of the presence of organochlorine compounds and polyaromatic hydrocarbon, suppression of acetylcholinesterase is an grounds of organophosphorus and carbamate pesticides, and initiation of metallothionein suggests the presence of heavy metals such as Cd, alterations in the Deoxyribonucleic acid and adducts formation is implicative of a genotoxic pollutant ( Walker et al. , 2001 ) .
This survey is to be carried out utilizing biospectropy attack. This comparatively new attack to non-destructive tissue probe has recorded little or no use in surveies undertaken in Nigeria. Information found in literatures supports this and has besides revealed the use and trust on tissue civilization, microscopy and histopathological techniques in tissue observation related surveies ( Obiorah and Nwosu, 2011 ) . Biospectroscopy enables us obtain biochemical finger prints of tissues which through computational analysis could be used to do sensible tax write-offs of the wellness position of the tissue under probe ( Kelly et al. , 2011 ) .
This attack offers a comparative advantage because it deals with tissues non-destructively and requires less sample readying for analysis. It is fast and cheap ( Llabjani et al. , 2012 ) . Consequences obtained in this attack compares really good with that obtained through histopathological techniques that involves tissue staining and microscopy.
AIMS AND OBJECTIVES
The aim of this survey is to utilize the biochemical finger prints obtained from fish tissue to set up the wellness position of the Ovia River. The usage of fish as a representative being highlights its importance and suitableness in the trophic degree. The economic importance of the river is rather priceless to the community and as such there is demand to hold information on the pollution position of the river.
Second, studies from surveies carried out within this part indicates a current diminution in certain aquatic species due to debut of anthropogenetic pollutants from industrial and domestic activities with attendant biochemical alterations of the aquatic organic structures ( Olomukoro and Ezemonye, 2006 ) . This of all time increasing anthropogenetic impact necessitates regular appraisal of the aquatic environments in order to better pull off aquatic resources. This survey is aimed at supplying scientific information that will increase bing cognition in this country and perchance function as consultable stuff and empirical groundss for policy devising.
Third, this research is aimed at placing the comparative consequence of different pollutants on the fish tissue for fish samples collected at different trying points so as to cognize the point of greatest impact and perchance happen out if such contaminations are traceable to agricultural or industrial wastewater discharge so that extenuating steps can be suggested.
Biospectroscopy technique would be used for this survey. It would be used for the bio-imaging of the tissue samples in order to obtain biochemical finger prints of the biomolecules of the tissue under probe. The fish would be dissected and relevant tissue ( bosom, liver, encephalon, sex glands, musculus, gills and kidney ) removed and fixed in paraffin and allowed to dry on glass slide. The tissue would be sectioned and stained with haematoxylin and eosin for histopathological scrutiny ( Krafft et al. , 2004 ) . The tissue would be scanned in Fourier Transform Infrared ( FTIR ) imaging system to obtain 32 scans for each image. Spectral information set obtained would be transformed utilizing a multivariate analysis for informations complexness decrease, categorization and reading ( Kelly et al. , 2011 ) . The image obtained relates the tissue architecture to absorbance spectra representative of biomolecules present in the tissue. The information obtained would be processed utilizing multivariate analysis which through computational analysis would ease the designation of biomarkers which can be used for diagnosing of morbid tissue
PROJECT MANAGEMENT AND TIME ALLOTMENT
This undertaking is planned to be undertaken within continuance of 16 hebdomads. Within this clip the different activities that constitute the undertaking will be undertaken with most of the agendas overlapping in order to maximize clip and every bit achieve stated aims. The undertaking commences with a field work which will be succeeded by sample processing, sample analysis, informations analysis and thesis authorship. It is worthy to advert that there is an ongoing background research which will go on in each of the agendas until the thesis is finished and submitted.
This research is expected to set up the wellness position of the river by finding the nature and categories of pollutants within. This is to be achieved by look intoing the biomolecular constituents of the tissue in order to detect alterations in its architectural morphology, histolopathological changes and utilize such in diagnosing of tissue pathology.
This research shall try to look into and infer possible long term consequence of pollutants on the aquatic species habitating the Ovia River so as to determine the ecological unity of the river.
This survey plans utilizing the ecological status of the river to mensurate public wellness as the presence or absence of pollutant in an aquatic community is contemplation of the human ambient environmental position.