Enzyme Activity Lab Essay

July 21, 2017 General Studies

Hypothesis:
1 ) If the disc is placed into each beaker with 100 units/ml of enzyme solution. so the clip for the disc to drift will be 30 seconds. 2 ) If the temperature of the solution is at 5 grades Celsius. so there will be less enzymes adhering to the active site. and if temperature is at 37 grades Celsius. so the reaction rate of the enzymes will be 3 times the old temperature’s reaction rate. 3 ) If the disc placed into each beaker of the substrate. so the clip for the disc to drift will take longer. like 1-2 proceedingss.

Discussion/Analysis:
1 ) How does enzyme activity vary with enzyme concentration? The rate of enzyme activity increases as the enzyme concentration additions. At 10 units/mL. the rate of enzyme activity was. 012 1/sec. and at 100 units/mL. the rate of enzyme activity was. 055 1/sec. 2 ) How is the rate of enzyme affected by increasing of substrate? The rate of enzyme is increased as the substrate concentration is increasing. At. 1 % of Hydrogen Peroxide. the rate is. 004 1/sec. and at. 5 % of Hydrogen Peroxide. the rate is. 015 1/sec. 3 ) What do you believe would go on if you increased the substrate concentration in this experiment? If the substrate concentration is increased. the rate of enzyme activity would increase every bit good. As show in the tabular arraies and graphs. the rate is increasing as the substrate concentration is increasing. 4 ) How does altering the substrate concentration comparison to altering the enzyme concentration in this experiment? Changing the substrate concentration has a greater difference than altering the enzyme concentration. For illustration. in the substrate concentration at. 01 % Hydrogen Peroxide. the rate is at. 004 1/sec and at 2. 0 % Hydrogen Peroxide. the rate is at. 070 1/sec. In the enzyme concentration at 10 units/mL. the rate is at
. 012 1/sec. and at 100 units/mL. the rate is at. 055 1/sec. 5 ) What are the two sorts of inhibitors and how do they move to forestall enzyme interactions? One sort of inhibitor is a non-specific inhibitor. which changes the chemical or physical province of the enzyme finally ensuing to denaturing the enzyme. The 2nd type is the specific inhibitor. which exerts their attempts on merely one enzyme. 6 ) From the temperature day of the month. what can you reason about how temperature affects enzyme action? How would you explicate these consequences? The different temperatures vary among the rates of enzyme activity. At 10 grades C. the rate is. 048 1/sec. But at 37 grades C. the rate decreases to. 032 1/sec. If it starts at 5 grades C. the rate will increase until it hits 37 grades C. so the rate will travel back down because the enzyme becomes denatured. 7 ) pH is another factor that can impact enzyme activity. What do you believe is the optimum pH for this enzyme and why? The optimum pH for this enzyme would be around 5-6. because murphies are somewhat basic. and for the enzyme to work to interrupt down the constituents. they have to be somewhat acidic.

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