Abstract: The Enzyme Lab results where when the liver was frozen, its reaction was fast, and when it was hot, it was slow, and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature, pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions, which would otherwise progress more slowly. (Background Information; pg. 1) pH is a measurement of the acidity or alkalinity (base) of a solution.
When the liver got mixed with H2O2 , the first time the chemical reaction was fast, the second time the reaction was slow and the last try was very fast. Temperature is the degree or intensity of heat present in a substance or object. When the temperature of the liver changed from freezing to very hot to room temperature, the reactions, obviously, changed. Hydrogen peroxide is the simplest peroxide and an oxidizer. When the liver combined with the H2O2 , like it was said before, the reactions varied. Concentration is defined as the abundance of a constituent divided by the total volume of a mixture.
The livers chemical reaction to the H2O2 , NaOH, and different temperature were interesting. If the liver is frozen then the reaction of H2O2 will be fast. And if the pH gets mixed with the NaOH and H2O2 then the reaction of the liver would be medium. Also if the surface area of the liver increases then the rate of the reaction would vary from slow to very fast. Experimental Design: Title: Enzyme Lab Hypothesis: If the liver is frozen then the reaction of H2O2 will be fast If the pH gets mixed with NaOH and H2O2 then the reaction of the liver would be medium.
If the surface area of the liver increases then the rate of the reaction would vary from slow to very fast. Independent Variable| Factor affecting enzyme activity | | | Levels Of Independent Variable| Temperature | pH| Concentration| Levels of levels of Independent Variable | Freezing Boiling (*C) (100*C)| 1 7 3| Room temperature Low High( *C)| Repeated Trials 1| 1 1 | 1 1 1 | 1 1| Dependent Variable| Rate of Reaction| | |
Constants| Same liver; Same amount of repeated trials; Same amount of time for each experiment. | | | Materials: 7 pieces of liver (room temperature) | 1 piece of liver(frozen)| 3 clean test tubes(for each experiment)| Hydrogen Peroxide(2mL)| Hot plate| Beaker| Graduated Cylinder| HCI(2mL)| NaOH(2mL)| Goggles | Lab coats| Stirring rod| Plastic Spoon| Mortar &Pestle| | | | | | | | | | | | | | | | | | | Procedure: Overall- In this lab, the liver(room temperature) reacted to the H2O2 fast. When the liver was put into the test tube ,frozen, the liver still reacted fast.
After the liver was in the boiling bath for 2 minutes the reaction of the liver was very slow. It had turned into a pink-tan color. The second experiment , the liver once again was put into a clean test tube. Put in 2 mL of H2O2 into the test tube. The liver then reacted at a medium rate. It then said to put 2 mL of HCI into the test tube. The liver reacted to the HCI slow. Then the NaOH was put in a new, clean test tube, along with a new piece of liver. The liver then reacted slowly again. For the last experiment the liver was crushed in the mortar.
Then the liver was scooped and put back in the test tube. Then the 2 mL of H2O2 was added. The liver reacted very fast. Data table: Enzyme Lab Conditions| | Rate | Of | Reaction| Best| | Very Fast| Fast| Medium| Slow| Reaction| pH(1,7,3)| | | 1| 2| 1. 5| Temperature(freezing 32*c, boiling point 100*c, room temperature 23*c)| | 2| | 1| 1. 5| Concentration(low, high)| 1| | | 1| 1| pH (1,7,3) Analysis/Discussion Parargraph 1: The purpose of this lab was for us to investigate which situations are most approving for the liver. It showed the students