Test for enzyme activity Lab Essay

October 22, 2017 March 26th, 2018 General Studies

Purpose:

The intent of this lab is to prove for enzyme activity. expression at enzyme specificity. and how temperature affects enzyme activity.

Hypothesis:

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I believe that the room temperature activity will bring forth the most activity.

Materials:

3 % H peroxide. a family liquidizer. murphies ( 3 medium size murphies. that are peeled and quartered ) . tap H2O. 8 trial tubings. 8 transportation pipets. sugar packages. salt. a little saucepan. a icebox with a deep-freeze. stove. 4 clear plastic cups. tongs ( to take trial tubings from boiling H2O ) . 4 java cups. cheesecloth to filtrate the murphy infusion. a wax pencil or Sharpie marker. a swayer. graduated table or balance. 250 milliliter graduated cylinder. and oven hand or tongs. Methods:1. Research ( online ) the reaction between catalase and H peroxide and be familiar with the reactants. merchandises. and enzyme. You will include the overall reaction in your lab study for this experiment. We will mensurate enzyme activity by mensurating the tallness of the bubbles produced.

2. Fix the murphy infusion ( catalase ) utilizing the undermentioned technique: – Peel 3 medium size murphies and cut them into quarters

-add the murphies to the liquidizer with 250 Master of Library Sciences of tap H2O to cover the murphies and intermix on high until the murphies are pureed ( blended to a thick liquid or paste ) – topographic point a cheesecloth over a container and utilize the cheesecloth to obtain the liquid part ( merely ) of your murphy puree. You can squash the cheesecloth in the terminal to obtain the concluding parts of the liquid from the puree. – Make a 1 % sucrose solution utilizing the method describe in your osmosis lab. – Make a 1 % salt solution by mensurating 1 gm of salt utilizing your graduated table or balance. add this to your graduated cylinder and so make full to the 100 milliliter grade. You can so reassign this mixture to a little saucepan. heat and splash until the mixture is dissolved.

Experiment 1: Testing for Enzymatic Activity

Measure 1:

Add 3mls of the undermentioned solutions to a labelled trial tubing utilizing a fresh transportation pipet with each new solution:

– trial tubing 1: pat H2O
– trial tubing 2: 1 % sucrose solution
– trial tubing 3: 1 % salt solution
– trial tubing 4: 3 % H peroxide


Measure 2:

Add 2 Master of Library Science of the catalase solution ( liquid part of the murphy infusion ) to each trial tubing and topographic point into a fictile cup to maintain it unsloped. Pick up each trial tubing. tap the bottom/ swirl the contents and so utilizing your swayer record in Table 1 the bubble tallness ( if any ) that is produced. Remember to include this tabular array ( with a strong rubric ) in your lab study.

Experiment 2- Testing the Effect of Temperature on Enzyme Activity Step 1: Label the trial tubes 1. 2. 3 and 4. where trial tubing 1 represents the sample placed on the counter at room temperature. trial tubing 2 represents the tubing placed in the icebox. trial tubing 3 represents the tubing placed in the deep-freeze. and trial tubing 4 represents the sample exposed to boiling H2O. You will expose catalase to each of these four conditions. Measure 2: Topographic point a saucepan with tap H2O on a burner and convey to a furuncle.

Measure 3:

Use the thermometer to place and enter the temperature for room temperature. in your icebox. in your deep-freeze. and so research the temperature of boiling H2O ( do non take this temperature ) and enter them in Table 2.

Measure 4:

Topographic point the trial tubing into separate java cups to keep the upward place. Add 2 Master of Library Science of the catalase solution to each of the trial tubing and so topographic point tubes1. 2. and 3 in the conditions described above. For trial tubing 4. fill the java mug half full of boiling H2O and so put trial tubing 4 in this container. Do non put the trial tubing into the pan of boiling H2O.

Measure 5:

Allow the tubings to sit for 5 proceedingss. so take the tubings from the conditions mentioned above ( note: be careful to utilize an oven hand or tongs to take the trial tubing exposed to boiling H2O from the java cup ) . Step 6: Then add 3 Master of Library Science of 3 % H peroxide. twirl all tubings. and mensurate the bubbles produced by mensurating bubble tallness ( if any are produced ) . Record this information in Table 2.

Consequences: Enzymatic Activity Test
Test Tube #
Solution
Height of Bubbles
1
tap H2O
1 centimeter
2
1 % sucrose solution
. 5 centimeter
3
1 % salt solution
. 1 centimeter
4
3 % H peroxide
2 centimeter














Temperature on Enzyme Activity
Test Tube #
Condition
Temp. of Condition
Sum of Hydrogen Peroxide
Height of Bubbles
1
Room Temp
76 F
3 Master of Library Science
. 5 centimeter
2
Electric refrigerator
50 F
3 Master of Library Science
. 3 centimeter
3
Deep-freeze
25 F
3 Master of Library Science
. 1 centimeter
4
Boiling Water
215 F
3 Master of Library Science
2 centimeter
























Decision:

The higher the bubbles. so the more enzyme activity is present. We are specially detecting the activity of enzymes being unnaturally produced. It is of import to utilize fresh transportation pipets for each new solution because if non so the lab will be inconclusive due to taint. My hypothesis was incorrect because I further investigate and found that room temperature does non better activity every bit much as boiling H2O.

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